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KMID : 1149220130210010177
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Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 1 p.177 ~ p.185
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Quantification Analysis and Antioxidant Activity of Leejung-tang
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Seo Chang-Seob
Kim Ohn-Soon
Kim Ye-Ji
Shin Hyeun-Kyoo
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Abstract
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Objectives : Leejung-tang (Lizhong-tang) has been used for treatment of gastrointestinal disorders in Korea. In this study, we performed quantification analysis of five marker components, liquiritin, ginsenoside Rb1, ginsenoside Rg1, glycyrrhizin, and 6-gingerol in Leejung-tang using a ultra performance liquid chromatography- electrospray ionization-mass spectrometer (UPLC-ESI-MS). In addition, we evaluated antioxidant activity of Leejung- tang.
Methods : The column for separation of five constituents used a UPLC BEH C18 (100 × 2.1 mm, 1.7 μm) maintained at 45°C. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in H2O (A) and CH3CN (B) by gradient flow. The flow rate was 0.3 mL/min with detection at mass spectrometer. The antioxidative activities conduct an experiment on 2,2＇-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of Leejung-tang.
Results : Calibration curves of five marker compounds were acquired with r2 values > 0.99. The amount of the five compounds in Leejung-tang were 0.07 ? 0.84 mg/g. The concentration required for 50% reduction (RC50) against ABTS radical was 119.02 ug/mL. In addition, the scavenging against DPPH radical of Leejung-tang was 11.4%, 14.5%, 19.8%, 29.6%, and 49.2% at 25 ug/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, and 400 μg/mL, respectively.
Conclusions : The established LC-MS/MS method will be helpful to improve quality control of Leejung-tang. In addition, Leejung-tang is a potential antioxidant therapeutic agent.
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KEYWORD
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Quantification Analysis, Antioxidant, Leejung-tang, Lizhong-tang, LC-MS/MS
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